1: Ex-Vivo signal Transduction Studies in Chronic Lymphocytic Leukemia Karly-Rai Rogers-Broadway, Laura Karydis, Rachel C Dobson, and Andrew J Steele 2: Ex vivo Pharmacological Profiling in Chronic Lymphocytic Leukemia Cells Burcu Aslan, Mary L. Ayres, and Varsha Gandhi 3: Culture and Harvest of CpG-stimulated Blood and Bone Marrow in Chronic Lymphocytic Leukemia Cecelia R. Miller and Nyla A. Heerema 4: Analysis of Common Abnormalities seen in Chronic Lymphocytic Leukemia using Fluorescence in situ Hybridization Reid G. Meyer and Daniel L. Van Dyke 5: Immunoglobulin Gene Analysis in Chronic Lymphocytic Leukemia Andreas Agathangelidis, Richard Rosenquist, Frederic Davi, Paolo Ghia, Chrysoula Belessi, Anastasia Hadzidimitriou, and Kostas Stamatopoulos 6: Detection and Functional Analysis of TP53 Mutations in CLL Sarka Pavlova, Jana Smardova, Nikola Tom, and Martin Trbusek 7: The Development and Use of Scalable Systems for Studying Aberrant Splicing in SF3B1-mutant CLL Tushar Murthy, Kiran V. Paul, Alexander C. Minella, and Manoj M. Pillai 8: Methods for Investigating VLA-4 (CD49d/CD29) Expression and Activation in Chronic Lymphocytic Leukemia and its Clinical Applications Antonella Zucchetto, Erika Tissino, Alexander Chigaev, Tanja Nicole Hartmann, and Valter Gattei 9: In vitro Assay to Study CLL and Monocyte Interactions Maria Teresa Sabrina Bertilaccio, Ronghua Zhang, Priyanka Banerjee, and Varsha Gandhi 10: Extracellular Flux Assays to Determine Oxidative Phosphorylation and Glycolysis in Chronic Lymphocytic Leukemia Cells Hima V. Vangapandu and Varsha Gandhi 11: Measurement of Leukemic B-cell Growth Kinetics in Patients with Chronic Lymphocytic Leukemia Andrea N. Mazzarello, Mark Fitch, Marc K. Hellerstein, and Nicholas Chiorazzi 12: Assays on DNA Damage and Repair in CLL Tzung-Huei Lai and Deepa Sampath 13: Method for Generating a Patient-Derived Xenograft Model of CLL Shih-Shih Chen 14: Immunological Methods to Study Monoclonal Antibody Activity in Chronic Lymphocytic Leukemia MJ Carter, LN Dahal, KLS Cleary, MJE Marshall, RR French, SA Beers, and MS Cragg 15: CRISPR/Cas9-based Gene Dropout Screens Kai Wu and Sami N. Malek 16: Gene Disruption using CRISPR-Cas9 Technology Nan Hu and Sami N. Malek 17: Minimal Residual Disease Quantification in Chronic Lymphocytic Leukemia: Clinical Significance and Flow Cytometric Methods Sebastian Böttcher 18: Rare Variant Quantitation Using Droplet Digital PCR Brian Parkin 19: Methods for Measuring ctDNA in Lymphomas Davide Rossi, Adalgisa Condoluci, Valeria Spina, and Gianluca Gaidano 20: Measurement of miRNAs in Chronic Lymphocytic Leukemia Patient Samples by Quantitative Reverse Transcription PCR Katrien Van Roosbroeck, Recep Bayraktar, and George A. Calin 21: Bioinformatics Analysis of Whole Exome Sequencing Data Peter J. Ulintz, Weisheng Wu, and Chris M. Gates 22: Genome Sequencing and Analysis Methods in Chronic Lymphocytic Leukemia Víctor Quesada, Miguel Araujo-Voces, José G. Pérez-Silva, Gloria Velasco, and Carlos López-Otín 23: Characterization of Somatically-acquired Copy Number Alterations in Chronic Lymphocytic Leukemia using Shallow Whole Genome Sequencing Helen Parker, Louise Carr, Sharma Syeda, Dean Bryant, and Jonathan C Strefford 24: High-throughput Sequencing of the T Cell Receptor Beta Chain Gene Repertoire in Chronic Lymphocytic Leukemia Vlachonikola E, Vardi A, Stamatopoulos K, and Hadzidimitriou A Skryť popis