Part I: Fundamentals of Using Light to Study Neocortical Dynamics

1. Neocortex in the Spotlight: Concepts, Questions, and Methods

Fritjof Helmchen and Bruno Weber

1. Principles and Fundamentals of Optical Imaging

Frank Scheffold

1. Optical Properties of Neural Tissue

Andrew K. Dunn

1. Fluorescent Indicators for Functional Optical Imaging

Walther Akemann, Hiroki Mutoh, and Thomas Knöpfel

1. Optogenetic Tools for Control of Neural Activity

Lief E. Fenno and Karl Deisseroth

Part II: Shedding Light on Cortical Activity States

1. In Vivo Dendritic Mapping of Sensory Inputs in Cortical Neurons

Hongbo Jia and Arthur Konnerth

1. In Vivo Population Imaging of Dendritic Integration in Neocortex

Masanori Murayama and Matthew E. Larkum

1. Voltage-Sensitive Dye Imaging of Cortical Dynamics

Carl C.H. Petersen

1. Two-Photon Imaging of Neuronal Network Dynamics in Neocortex

Benjamin F. Grewe, Fritjof Helmchen, and Björn M. Kampa

1. Chronic Two-Photon Imaging of Neural Activity in the Anesthetized and Awake Behaving Rodent

David J. Margolis, Henry Lütcke, Fritjof Helmchen, Bruno Weber, and Florent Haiss

1. Neocortical Circuit Interrogation with Optogenetics

Lief E. Fenno and Karl Deisseroth

Part III: Elucidating Metabolic States of Cortical Circuits

1. Imaging of Astrocytic Activity in Living Rodents

Norio Takata, Yoshiaki Shinohara, Masamichi Ohkura, Tsuneko Mishima, Junichi Nakai, and Hajime Hirase

1. Imaging Morphology and Function of Cortical Microglia

Bianca Brawek and Olga Garaschuk

1. Functional Imaging of Cerebral Oxygenation with Intrinsic Optical Contrast and Phosphorescent Probes

Anna Devor, Sava Sakadzic, Mohammad A. Yaseen, Emmanuel Roussakis, Peifang Tian, Hamutal Slovin, Ivo Vanzetta, Ivan Teng, Payam A. Saisan, Louise E. Sinks, Anders M. Dale, Sergei A. Vinogradov, and David A. Boas

1. Laser Speckle Imaging of Cerebral Blood Flow

Pavel Zakharov, Frank Scheffold, and Bruno Weber

1. Two-Photon Microscopy to Measure Blood Flow and Concurrent Brain Cell Activity

Andy Y. Shih, Jonathan D. Driscoll, Michael J. Pesavento, and David Kleinfeld