Raman imaging of PMMA particles in aqueous environment was successfully performed using two different Raman set-ups: the confocal Raman microscope (Horiba Jobin-Yvon LabRAM 800HR-633nm) and a Raman probe optimized for process monitoring (Kaiser Optical Systems RXN-1-785 nm). Herein, the developed technique allowed to obtain higher signal intensity of the Raman spectrum for micro-sized particles in suspension. While there are hardly few Raman bands of particles visible in the spectra obtained with conventional Raman spectroscopy. Here, i showed one way to overcome this limitation by hyphenation of Raman spectroscopy with ultrasound particle manipulation. The spectroscopic fingerprint of the particles are perfectly visible once the ultrasound has been turned on. Skryť popis