Part I: PROTEIN ANALYTICS Protein purification Protein determination Enzymatic activity tests Microcalorimetry Imunological Techniques Cheminal modification of proteins and protein complexes Spectroscopy Light microscopy - Imaging Cleavage of proteins Chromatographic techniques Electrophoretic techniques Capillary electrophoresis Amino acid analysis Protein seqeunce analysis Mass spectrometry Protein protein interactions The Two-Hybrid System TAP-Tagging and Purification of Protein Complexes Analyzing Interactions In Vitro: GST-Pulldown Surface Plasmon Resonance Spectroscopy Fluorescence Resonance Energy Transfer (FRET) Analytical Ultracentrifugation Analytical Ultracentrifugation Biosensors Part II: 3D STRUCTURE DETERMINATION NMR EPR Electron microscopy Atomic force microscopy X-ray structrure analysis Part III: PEPTIDES, CARBOHYDRATES, AND LIPIDS Analysis of synthetic peptides Carbohydrate analysis Lipid analysis Analysis of posttranslational modifications Part IV: NUCLEIC ACID ANALYTICS Isolation and Purification of Nucleic Acids Analysis of Nucleic Acids Restriction analysis Electrophoresis LC-MS of Oligonucleotides LC-MS of Oligonucleotides Techniques for the Hybridization and Detection of Nucleic Acids Polymerase chain reaction DNA sequencing Analysis of epigenetic modifications Protein nucleic acid interactions Part V: FUNCTIONAL AND SYSTEMS ANALYTICS Sequence analysis by bioinformatics Analysis of promotor strength Hybridization of fluorescent DNA for genome analysis in molecular cytogenetic Physical and genetic mapping of genomes DNA microarray techmology The Use of oligonucleotides as tools in cell biology Proteome analysis Metabolomics and peptidomics Interactomics - systematic protein protein interactions Chemical Biology Toponome Analysis Mass Spectrometric Imaging Skryť popis