1. Visualization and Measurement of Multiple Components of the Autophagy Flux Tracey Evans, Robert Button, Oleg Anichtchik, and Shouqing Luo 2. Mitochondrial Redox Sensor for Drosophila Female Germline Stem Cells Kiran Suhas Nilangekar and Bhupendra V. Shravage 3. Induction and Detection of Autophagy in Aged Hematopoietic Stem Cells by Exposing Them to Microvesicles Secreted by HSC-Supportive Mesenchymal Stromal Cells Rohan S. Kulkarni, Manmohan S. Bajaj, and Vaijayanti P. Kale 4. Skeletal Muscle Lysosomal Function via Cathepsin Activity Measurement Kristyn Gumpper, Matthew Sermersheim, Michael X. Zhu, and Pei-Hui Lin 5. Autophagy in Adipocyte Differentiation Zhipeng Tao, Longhua Liu, Louise D. Zheng, and Zhiyong Cheng 6. Assessing Autophagy in the Leydig Cells Hui Gao, Chao Liu, and Wei Li 7. Determination of Autophagy in the Caco-2 Spontaneously Differentiating Model of Intestinal Epithelial Cells Sinem Tunçer and Sreeparna Banerjee 8. Simultaneous Detection of Autophagy and Epithelial to Mesenchymal Transition in the Non-Small Cell Lung Cancer Cells Javad Alizadeh, Shahla Shojaei, Adel Sepanjnia, Mohammad Hashemi, Eftekhar Eftekharpour, and Saeid Ghavami 9. Autophagy in Zebrafish Extraocular Muscle Regeneration Alfonso Saera-Vila, Phillip E. Kish, and Alon Kahana 10. The Detection Techniques for Autophagy-Associated Cell Death Related Genes and Proteins: Gene Expression Assay and Immunohistochemistry Melek Ozturk, Ozlem Dagdeviren Ozsoylemez, and Fatma Kaya Dagistanli 11. Cloning of Autophagy-Related MicroRNAs Deniz Gulfem Ozturk, Muhammed Kocak, and Devrim Gozuacik 12. Assays to Monitor Aggrephagy in Drosophila Brain Anne-Claire Jacomin and Ioannis P. Nezis 13. Methods for Monitoring Autophagy in Silkworm Organs Aurora Montali, Morena Casartelli, Daniele Bruno, Annalisa Grimaldi, and Gianluca Tettamanti 14. Immunofluorescence Staining Protocols for Major Autophagy Proteins Including LC3, P62, ULK1 in Mammalian Cells in Response to Normoxia and Hypoxia Wen Li, Shupeng Li, Yifang Li, Xiaoying Lin, Yongquan Hu, Tian Meng, Baojin Wu, Rongrong He, and Du Feng 15. Identification of Novel Autophagy Inhibitors via Cell-Based High-Content Screening Georgios Konstantinidis, Sonja Sievers, and Yao-Wen Wu 16. Porcine Cell-Free System to Study Mammalian Sperm Mitophagy Won-Hee Song and Peter Sutovsky 17. Monitoring and Measuring Mammalian Autophagy Sujit K. Bhutia, Prakash P. Praharaj, Chandra S. Bhol, Debasna P. Panigrahi, Kewal K. Mahapatra, Srimanta Patra, Sarbari Saha, Durgesh N. Das, Subhadip Mukhopadhyay, Niharika Sinha, Prashanta K. Panda, and Prajna P. Naik Skryť popis